Amplification of the purine-rich region from upstream of miRNA 17-92a sequence for triple-helix study / Nur Amira Jemal @ Zainal

In the human body, there are the existence of sequences that are specific for the formation of triple helix. The triplex-forming oligonucleotides (TFO) might be very useful therapeutically for example in suppressing cancer. This review generally describes the process of generating the template for the TFO binding site. A very short sequence of template which is 18bp from the hepG2 cancer cell line were amplified using the specially designed primers by polymerase-chain reaction (PCR). Amplifying such short sequence may encounter problem like the formation of secondary structures such as primer dimer and hairpin loop. The desired region of PCR product was purified and sent for direct sequencing to verify the sequence. This study encourages further steps and processes that need to be done to study on TFO.