Qualitative analysis of banana soft pith crude extract anti-proliferative properties against human colorectal adenocarcinoma cell lines (HT 29) / Siti Nur Nadiah Mohamad Alwi

Colorectal cancer (CRC) is one of the worldwide killer diseases. Several treatments have been implemented to treat cancer patients such as chemotherapy and surgical. Nevertheless, the side effects from the treatment causing the patients to possibly suffer low quality of life as the outcome. Previous study of BSP crude extract against Human Colorectal Adenocarcinoma Cell lines (HT 29) showed the cytotoxic effect of BSP crude extract. In this study, AO/PI and TEM method were used to observe the morphological changes of cell structure in treated samples. 6 different concentrations were used for AOPI staining technique which were 0, 50, 100, 500 and 1 OOOµg/ml and incubated for 24, 48, 72 hours. Further verified by TEM method, IC50 of BSP crude extract was used to treat CRC cells after 72 hours incubation. The results were able to verify the morphology changes of treated HT 29 such as membrane blebs, fragmented nuclei, cell shrink, chromatin condense, secondary necrosis and apoptotic body. In conclusion, BSP crude extract was found to induce apoptotic features of HT 29 cell lines.