Development of a PCR based method to detect polymorhism of adipoq / Nurul Syafiqah Samsuri

Adipnectin is an adipose tissue- specific protein which exists in the circulation of healthy individual. It is encoded by ADIPOQ gene which is located on chromosome 3q27 and plays important role in regulating the insulin sensitivity of individual. Polymorphism of this ADIPOQ means that there is variation in the nucleotide sequence of ADIPOQ gene due to substitution variation. This polymorphism leads to reduction in insulin sensitivity in regulating the blood glucose level in the individual. This causes the inability of the cells to convert excess glucose to another form and lead to chronic disease which is Type 2 Diabetes. The aim of this study is to develop a PCR based method to detect the polymorphism of ADIPOQ gene. Primers were designed according to the gene and followed by reconstitution of primers working stock. Besides that, different DNA samples were used in order to detect the polymorphism by using PCR method. Based on the results of this study, it can be concluded that Malaysian population might carry the heterozygous genotype which means they carried the mutant allele for Type 2 Diabetes. It is suggested that for future studies, larger sample sizeare used in order to verify the preliminary finding of this project.