Isolation of actinomycetes of rubiaceae plants from UiTM Puncak Alam biological reserve / Syazana Asmira Abdullah

Isolation of actinomycetes from the terrestrial environment has been widely investigated. Choosing a unique sources or an under investigating plants species may increase the chances of finding actinomycetes. The isolation of endophytic actinomycetes from surface-sterilized tissue of four plants species from Rubiaceae family labelled as THI, TH2, TH3 and TH4 was made using three types of non­ selective medium i.e potato-dextrose agar, nutrient agar and 25% of agar in water and six types of selective medium for Streptomyces i.e ISP 1, ISP 2, ISP3, ISP4, TSP5, and ISP 7. Out of 5 isolates discovered, 1 was from leaves, 2 were from stem and 2 were from roots. A microscopic examination of these five isolates was done by using light microscope revealed that they own certain morphological characteristics of an actinomycetes. Two out of five crude extract of isolates were further analysed by HPLC profiling analysis and the chromatogram of TH4.A sample from root showed that two peaks at retention time of 22.833 and 24.437 were significantly different from the standard chromatogram.