Antibacterial activity of flavonoids and petroleum ether extract of hibiscus rosa-sinensis leaves / Aisyah Amanina Abdullah Salim

The study was done to determine the antibacterial effect of flavonoids and petroleum ether extract of Hibiscus rosa-sinensis leaves in Staphylococcus aureus (S. aureus), methicillin-resistant Staphylococcus aureus (MRSA), Streptococcus agalactiae (S. agalactiae), Streptococcus pyogenes (S. pyogenes), Pseudomonas aeruginosa (P. aeruginosa), Escherichia coli (E. coli), Klebsiella pneumonia (K. pneumonia) and Shigella spp. Evaluation of antibacterial activity was carried out using broth microdilution assay. MTT assay was used to determine the minimum inhibitory concentration (MIC). The results showed the MIC values of rutin were 10 mg/ml against S. aureus and K. pneumonia and 1 mg/ml against S. agalactiae and S. pyogenes. The MIC values of naringenin were 1 mg/ml and 0.05 mg/ml against S. agalactiae and S. pyogenes respectively. The MIC value of kaempferol, taxifolin and myrecitin against S. pyogenes was 0.05 mg/ml. The MIC value of petroleum ether extract of Hibiscus rosa-sinensis leaves against S. aureus was 5 mg/ml. There were no MIC values determined in MRSA, P. aeruginosa, E. coli and Shigella spp in both of flavonoids and petroleum ether extract of Hibiscus rosa-sinensis leaves. This study showed positive effect of flavonoids; rutin, naringenin, kaempferol, taxifolin and myricetin and petroleum ether extract of Hibiscus rosa-sinensis leaves as antibacterial properties.