Effects of endophytic aspergillus sp. strain HAB10R12's fractions (R1 and R2) on TNF-α induced insulin resistance in differentiated 3T3-L1 adipocytes / Nurul Rahimah Mohd Khair

Insulin is responsible for regulation of glucose uptake and circulating free fatty acids (FF A). When there is insulin resistance, there will be an increased in circulating FF A concentrations and fat accumulation that impede insulin mediated glucose in skeletal muscle and elevated glucose production in liver. This will lead to the development of metabolism problem including Type 2 diabetes which can cause other serious problem such as cancer. Therefore, it is important to find a compound that able to reverse insulin resistance. There is growing evidence that bioactive substance produced by microbial endophytes have applicability in medicine, agriculture and industry . So, the aim of this research is to study the effect from fraction of endophytic Aspergillus sp. strain HAB10R12 (RI and R2) on normal 3T3-L1 adipocytes and insulin resistance 3T3-L1 cell induced by TNF-a. Results in MTT Assay test shown that RI and R2 treatments caused no cytotoxic effects to the normal 3T3-L1 cells and no IC50 value observed in the data. Besides, in glucose uptake test, when compared with the untreated insulin resistance cell, there was in increased in percentage of glucose uptake by insulin in the insulin resistance cell treated both RI and R2 treatments from the lowest concentration up to 0.01 µg/ml.