Isolation and identification of oligostilbenoid from neobalanocarpus heimii (dipterocarpaceae) / Mohd Fazirulrahman Fathil

The leaves of Neobalanocarpus heimii was investigated for their oligostilbene contents. The powdered material was obtained from previous master students and was used for the research. Prior to isolation using HPLC, determinations of compounds for isolation were based on mass spectrometry. The method employed for the research is based on the established method and was modified to comply with different HPLC techniques such as preparative and analytical HPLC. The crude sample was injected into preparative HPLC and obtains two fractions which one of them is pure and another is impure. The impure fraction was further isolated using analytical HPLC and obtain two pure fraction. The pure fractions then were characterized using nuclear magnetic resonance (NMR). One galactopyranose and one oligostilbene, balanocarpol were identified.