Acute toxicity of a novel synthesized stilbene / Eni Fazihan Ismail

Stilbene is a naturally and synthetically compound with have several biological activities such as antioxidants, anti-inflammatory, anticancer, antibacterial and antifungal agent. The toxicity of newly synthesized compound were determined by using in vivo studies before being approved as a safety compound to prevent damage or any adverse effects to the vital organ in the body. However, acute toxicity test normally carried out with definite order by choosing the critical dose range in order to investigate the lowest dose that induces any side effects and toxicity and the highest dose possible that do not show any effect to the tested animal. Therefore, two different groups of mice were used to determine the acute toxicity of newly synthesized stilbene. These studies were conducted at Laboratory Animal Facility & Management (LAF AM) and Pharmacology Laboratory at Universiti Teknologi MARA (UiTM) in Puncak Alam campus. The primary objective of the study is to determine the acute toxicity of the 3,4,10- trimethoxystilbene on male and female albino mice. There are several parameters were used to determine the toxicity of the compound. During 14 days of treatment, the result were observed for changes in clinical sign and mortality included behaviour changes of the treated mice before and after administration of doses, thus the comparison of body weight, water intake and food intake for all groups of mice were did not show any significant difference (p < 0.05) between controlled and treated group. After 14 days of the treatment, both groups of controlled and treated mice were sacrifices via cardiac puncture. The collected blood were centrifuge to collect the serum of the blood for biochemical analysis using ILAB machine to determine the level of ALT, AST, ALP, cholesterol and urea presence in the blood. This parameter were used to determine the toxicity of the compound by comparing between treated and control group of the mice, the result shows there is no significant difference (p < 0.05) between both groups. However, the organ that removed for gross necropsy were undergo histology of organ tissue where several step involved before getting complete staining slice and observed under light microscope to examined any abnormal changes related with the mice tissue structures when treated with stilbene compound. Therefore, the result shows no significant difference (p < 0.05) regarding the severity of tissue changes between controlled and treated group of mice. The study give more information in determine the toxicity studied involving newly synthesized compound.