Cytotoxicity of component in rebatea tarik at 24 hours observation in WRL-68 cells / Nurul Diyana Kamarulzaman

As we know, 'tea tarik' is one of the cultures in Malaysia. However, high calories and sweet taste of 'tea tarik' draw public concern for adverse health effects as it is prone to cause obesity and diabetes. Thus, RebA Tea tarik is produce to overcome these circumstances. RebA Tea tarik is expected to be a healthy and tasty tea tarik formulation which is safe to be consumed by the 'tea tarik' lover eventhough patient with diabetes. Therefore, this research project is performed to determine cytotoxicity of different component in RebaTea tarik at 24 Hours observation in normal human hepatic cells (WRL-68 cells). WRL-68 cells were treated with six concentration of different component in RebA Tea tarik and cytotoxicity was measured by using MTT (3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay. After 24 hours observation, all cells treated with rebaudioside A, skimmed milk, tea extract, and RebA Tea Tarik formulation showed low cytotoxicity level. Increasing pattern of cell viability was demonstrated by cells treated with skimmed milk which was above 100% while decreasing pattern was observed with cells treated with rebaudioside A which was not less than 82%. The cytotoxicity level of all components did not fall below than 70% cell viability. Based on this study, it can be concluded that component in RebA Tea tarik did not exhibit any harm or toxic to WRL-68 cells and this formulation is safe to be consumed by the 'tea tarik' lover.