The effect of local fertilizers on growth of bulbophyllum fascinator and dendrobium cretaceum protocorms / Nur Fariha Yaakob

Currently orchids industrial get a high demand from the global market. Plant tissue culture technique provides an alternative ways to propagate orchid protocorms effectively compared to conventional method. Nowadays, researchers have manipulated basal medium by incorporating different materials to enhance the growth of orchid protocorms. Therefore, the aim of this study is to investigate the influence of two different local fertilizers, namely, Gaviota and B 'GREEN Seaweed fertilizer on the growth of Bulbophyllum fascinator and Dendrobium cretaceum protocorms. The protocorms were cultured on the half strength Murashige and Skoog(MS) basal medium supplemented with 30 g/1 sucrose, 2 g/l Gelrite agar , 1 g/1 charcoal and various concentrations of Gaviota and B'GREEN Seaweed fertilizer. For the media that incorporated Gaviota as a fertilizer, the most optimum growth of Bulbophyllum fascinator was observed on Media I (Y2 MS added with 150 mg/1 of Gaviota fertilizer). The most optimum growth of Dendrobium cretaceum was observed on Media L (Y2 MS added with 300 mg/1 Gaviota). For media using B'GREEN Seaweed as a fertilizer, it was observed on Media A (Yi MS added with 1 ml/1 B'GREEN Seaweed fertilizer) showed the positive influence on the growth of Bulbophyllum fascinator and Dendrobium cretaceum protocorms. Based on the observation, the growth of these protocorms was retarded on Media F (Y2 MS added with 6 ml/I B'GREEN Seaweed Fertilizer).