In-vitro anti-inflammatory activity of standardized aqueous extract of erythroxylum cuneatum in lipopolysaccharide-induced RAW 264.7 cells / Siti Khuzaimah Maarof

Erythroxylum cuneatum has long been used by the folks as a traditional herbal medicine to treat inflammatory disease such as rheumatoid arthritis by chewing the dried leaves. This plant was chosen because only few studied was conducted on Erythroxylum cuneatum. The aim of the study is to determine the in-vitro anti­ inflammatory activity of standardized aqueous extract of Erythroxylum cuneatum in lipopolysaccharide-induced RAW 264.7 cells. We evaluate the protective and toxicity effect of Erythroxylum cuneatum by measuring the percent cell death in MIT assay. The pro-inflammatory cytokines such as Tumor Necrosis Factor alpha (TNF-a) and Interleukin- I a (IL- I a) production were evaluated after the cell were induced by the lipopolysaccharide (LPS) of gram negative bacteria with presence of Erythroxylum cuneatum. The pro-inflammatory cytokine were evaluated by ELISA. In this research, upon stimulation of Erythroxylum cuneatum in lipopolysaccharide-induced RAW 264.7 cell showed that there are significantly reduced of the amount of pro-inflammatory cytokines production. These results suggest that Erythroxylum cuneatum may have anti­ inflammatory activity by inhibiting TNF-a and IL-1 a in RAW 264. 7 cell.