Chromatographic study of the ethanol extract of fagopyrum species / Shuaiani Hussainar

Fagopyrum esculentum and Fagopyrum tartaricum are important crops in many countries due to its multipurpose benefits in agriculture and medicinal fields. This research was carried out to review the biological properties of Fagopyrum esculentum and Fagopyrum tartaricum, to extract both of these plants using ethanol and investigate the compounds that could be detected in these plants. From the literatures, Fagopyrum esculentum exhibits anti-oxidant properties besides of suppressing weeds. Fagopyrum tartaricum exhibits more biological properties than Fagopyrum esculentum. Fagopyrum tartaricum has the properties of anti-tumor, anti-infection, anti-fatigue and anti-oxidant. The ethanol extracts of both samples were subjected to Thin Layer Chromatography (TLC) however only Fagopyrum esculentum extract was subjected to preparative TLC and Nuclear Magnetic Resonance (NMR) spectroscopy. The mobile phase system of hexane: ethyl acetate: acetic acid (85:10:5) showed good results for TLC for both extracts. More spots were observed in the Fagopyrum esculentum extract rather than Fagopyrum tartaricum extract using the mentioned mobile phase system. The NMR spectrum of Fagopyrum esculentum extract revealed fatty acids as its composition. The fatty acids that were detected were oleic and linolenic acids. This study met the objectives of this research. In conclusion, the objectives were successfully achieved by reviewing the scientific articles, utilizing chromatographic procedures and investigating the spectroscopic data.