Effects of crude and fraction (C3) endophytic aspergillus sp. HAB10R12 on the TNF-α induced - insulin resistance differentiated 3T3-L1 adipocytes / Nur Amalina Yahaya

Endophytes Aspergillus sp strain HAB10R12 isolated from tree Garcinia scortechinii (kenondong krot @ kandis) exert many beneficial effects such as anticancer, antimicrobial, and anti-diabetic. However, the study on the antidiabetic of this compound is still limited. In the present study, we analysed the possible effect of the Endophytes Aspergillus using the 3T3-L1 cells as an in vitro cellular model. MTT assay has confirmed that the endophyte extracts do not cause cytotoxic effect on 3T3-L1 adipocytes since there is no IC-50 after 24 hours exposure. However, endophytes may or may not stimulate the glucose uptake 3T3-L1 adipocytes. The observation on glucose uptake demonstrated that endophytes had varying ability to take up glucose in the insulin resistance induced 3T3-L1 adipocytes with TNF.