Cytotoxicity study of banana soft pith (BSP) of musa paradisiaca acetone extracts against human colon adenocarcinoma cell line (HT29) / Nurul Izwa Mansor

Banana soft pith (BSP) anti-cancer properties are yet to be studied and its therapeutic value is still lacking of clinical evidence. BSP has been traditionally being consumed as food and medication purposes. Previously, our colleagues found that BSP extract possesses anti-proliferative effects towards liver cancer cell line (HepG2) and breast cancer cell line (MCF7). The aim of this study is to investigate the anti-cancer potential of acetone extract of BSP (Musa Paradisiaca cv Kebatu) towards human colon adenocarcinoma cell line (HT29). The study was conducted by performing MTT assay and microscopic analysis. The cells were subjected to treatment with various concentrations (0, 50, 100, 250, 500, and 1000µg/ml) and treated for 24, 48 and 72 hours. The results obtained showed percentage of cell viability was significantly reduced to 7 .35% (p<0.05) after being treated with 1000µg/ml sample for 72 hours incubation. The IC50 obtained was 229µg/ml. The microscopic analysis qualitatively showed that the cells were reduced in cell size and cell number. These finding suggest that acetone extract of BSP posses anti-proliferative properties. Therefore, further studies of this extract shall be continued to further understand the underlying mechanisms.