Identification of differentially expressed genes in a lung cancer cell line, A549 treated with gelam honey / Siti Nazurah Mohammad Seth

Lung cancer has become one of the most deadly cancers in the world. One of the main causes of cancer is reactive oxygen species (ROS) in which it causes the increase of oxidant as compared to antioxidant. This imbalance can contribute to cancer pathogenesis. This study aims to know the differentially express gene of lung cancer cell line, Human Lung Adenocarcinoma Epithelial Cell Line 549 (A 549) when being treated with Gelam honey. Gelam honey is chosen in this research as it contains high amount of flavonoid and phenolic compounds which act as antioxidants and free radical scavenger. The amplification of lung cancer cell line A 549 by using GeneFishing™ DEG Premix Kit shows the down-regulated gene of cell treated with Gelam honey as compared to the untreated cell. The gene was successfully clone by using TOPO TA Cloning® Kit for Sequencing to identify the differentially express gene.