Preliminary screening of prebiotics on lactobacillus plantarum L9 growth capability / Nora Awang Jalil

Lactobacillus plantarum (L. plantarum) is one of the probiotics that widely studied since the fact that, it has copious potential health benefits. In this study, growth L. plantarum was studied by addition of prebiotics and non prebiotics such as com starch, gum Arabic and glucose. The fermentation of L. plantarum was done in shake flasks and incubated aerobically at 37°C for 24 hours and the result was measured by optical density at 620nm wavelength (OD620) using spectrophotometer. The fermentation media was prepared for 15 runs with different concentrations of prebiotics and non-prebiotic by following three factorial Box-Behnken designs. The principle of this study is to screen prebiotics by investigate the main and interaction effect on the cell growth of L. plantarum. In this experiment, L. plantarum fermentation revealed growth patterns which include lag phase, exponential phase, stationary phase and death phase. Based on the results, maximal optical density of L. plantarum was 0.226 OD620 which contain high proportion of com starch. However, referring to specific growth rate of L. plantarum, it was high when the maximum presence of gum Arabic compared to com starch. For further results, analysis of variance (ANOVA) indicated that all prebiotics and · non-prebiotic had no significance difference (p>0.05) on growth capability of this strain. Response surface methodology (RSM) predicted optimum concentrations of prebiotics, only concentration of gum Arabic and glucose was 4.8% and 4.0% respectively will gives significant and maximal OD620 (0.270). It is suggested that in future study, the range of selected prebiotics and probiotics should be diverse, thus experimental data would be more precise and reliable for future references.