Antioxidant properties ofwater soluble polysaccharides from the shoots of buluh beting (Gigantochloa levis) / Nurul Daniela Azman

The aims of this study are to characterize water soluble polysaccharide from the shoot of Buluh beting (Gigantochloa levis) and to determine the antioxidant properties as well as chemical composition it contains.The hot water extraction methodproduced about 2.5±0.02 % of water soluble polysaccharide in dry basis. Investigations showed that the extract contained 4.46±0.02 % of moisture, 3.45±0.08 % of ash, 0.01±0.01 % of protein, 20.1±0.11 % of glucose and 96.15 % of solubility. The antioxidant properties tested for the water soluble polysaccharides extracted were Total Phenolic Content (TPC), 1,1-diphenyl-2-picrylhydrazyl (DPPH) and Ferric Reducing Antioxidant Power (FRAP). For the TPC measured by FolinCiocalteau methods the phenolic amount obtained was 4.44 mgGAE/100 g extract. The FRAP conducted resulted in84.14±2.93mgTE/g. For the DPPH analysis, 96.16±0.04 % scavenging effect was evaluated. As a conclusion, the synthetic antioxidant (BHA and BHT) was found higher in antioxidant properties than the natural antioxidant properties contained in the shoots of Buluh beting (Gigantochloa levis).