Secondary metabolites and antioxidant properties of selected mangrove isolates of actinomycetes / Rojilin Donol

This study reports the successful isolation and bioactivity of mangrove actinomycetes. Four selected actinomycetes were isolated from soil samples collected from Binsulok, Beaufort. Four selected isolates of actinomycetes were assessed for presence of secondary metabolites and antioxidant properties. All isolates were fermented for 10 days at room temperature (28 °C) in Muller Hinton broth (MHA) with 7 ± 0.5 at 28° C for detection of organic compound and the level of antioxidant potential by DPPH scavenging activity. The screening method detects for secondary metabolite which were saponin, flavonoids. alkaloids and tannin. The antioxidant activity was determined by DPPH scavenging activity. The data from DPPH assay showed that four selected isolates have antioxidant properties, which were, R - 1 (85.13%), R-2 (90.28%), R-3 (76.74%) and R-4 (77.62%). The selected isolate of mangrove's actinomycetes (R-2) which was 90.28% showed the highest of antioxidant activity but the R-3 and R-4 had the strongest antioxidant activity. The ability of actinomycetes produced bioactive compound depends to environment state. Further study on these selected isolated actinomycetes should be done more in term of identification and confirmation of actinomycetes species, determination of active compound and different concentration of bacteria extract.