The generation of CYP2C9 knocked-down human hepatocyte library: a utilization of crispr/cas9 system / Ruhil Nadirah Che Omar

There is an issue of inconsistencies of working with hepatocytes obtained from different human liver donors in drug metabolism studies. CRISPR type II (Cas9) is the most recent development in biotechnology that could potentially resolve this limitation by precise and efficient alteration of genetic sequences. It works in almost any kind of living cells and became possible after the recent discovery of genome editing technologies. Clustered regularly interspaced short palindromic repeats (CRISPR) – associated nucleus (Cas), allows for small changes to a known, targeted location on the DNA sequence efficiently at a faster rate, and ease of use.