Hormonal changes in female bisphenol A treated sprague dawley rats at different time response / Mohd Faiz Bin Mohd Yazid

Bisphenol A (BPA) is one of the endocrine disrupting chemical due to its capacity to mimics or interfere with several hormones, including progesterone. It is widely used in the production of polycarbonate plastics and epoxy resins. In this study we investigated the effect of BPA exposure to progesterone level in Sprague Dawley (SD) rats for different time response (7, 14 and 28 days). Nine groups of rats were prepared for negative control, positive control and BPA treated with seven, fourteen and 28 days duration of treatment. Positive control groups were treated with 1.0 µg/kg/day ethinyl estradiol (EE) while BPA group were treated with 100 mg/kg/day of BPA via force feeding. Daily weight of the rats were recorded and the plasma level of progesterone was examined by using ELISA method. Independent-groups t­ test was then performed. The result showed that the progesterone concentration was not normally distributed and therefore, it is suggested that there was no significant differences of progesterone level in each treated groups (P>0.05). For the weight gain, there is no significance difference for seven days of treatment. Weight gain was significance between the negative and positive control group for fourteen days and 28 days of treatment respectively.