The effect of inducer concentration on the activity of recombinant phospholipase A2 enzyme in escherichia coli / Muhaini Abd Hadi

Abd Hadi, Muhaini (2009) The effect of inducer concentration on the activity of recombinant phospholipase A2 enzyme in escherichia coli / Muhaini Abd Hadi. Degree thesis, Universiti Teknologi MARA (Kampus Puncak Alam).

Abstract

Phospholipase A2 is used widely in food, cosmetics and pharmaceutical industry. The recombinant PLA2 previously was constructed in order to overcome the issue of halal status among Muslim consumer. Arabinose is inducer that plays important role in protein expression for recombinant using pBAD as promoter. Thus different concentration of Arabinose which is 0.2% and 0.02% was used to study the effect of concentration of Arabinose on the activity of recombinant PLA2 in E.coli. The test was done by running SOS-Page and Microassay analysis. The result shows that of 0.2% arabinose produce high rate of activity on pBADffOPO PLA2 in E.coli clone 5 while 0.02% significant for pBAD/TOPO PLA2 in E.coli clone 8.

Metadata

Item Type:	Thesis (Degree)
Creators:	Creators Email / ID Num. Abd Hadi, Muhaini UNSPECIFIED
Contributors:	Contribution Name Email / ID Num. Thesis advisor Mohd Joffry, Suhaidah UNSPECIFIED
Subjects:	H Social Sciences > HD Industries. Land use. Labor > Special industries and trades > Pharmaceutical industry R Medicine > RS Pharmacy and materia medica > Materia medica > Pharmaceutical chemistry
Divisions:	Universiti Teknologi MARA, Selangor > Puncak Alam Campus > Faculty of Pharmacy
Programme:	Bachelor of Pharmacy
Keywords:	inducer concentration, Phospholipase A2 enzyme, escherichia coli
Date:	2009
URI:	https://ir.uitm.edu.my/id/eprint/107055