Gene expression changes in male reproductive system of dose response, BPA-treated sprague dawley rats / Shazwani Zulkifli

Bisphenol A (BPA) is a xenobiotic extrogenic compound which has been suspected to have estrogenic effects on reproductive system of males and females. This study is conducted to investigate on physical and gene expression changes of male hormones in male SD rats after administration of different dose of BP A. Six groups of SD male rats (post natal day 28) were used and four groups among them were administered daily orally with 50µg/kg body weight/day, 500µg/kg body weight/day, 1000 µg/kg body weight/day and 5000 µg/kg body weight/day. The other groups were negative control and positive control. In this study, RNA samples have been isolated and amplified using Reverse Transcriptase Polymerase Chain Reaction (RT PCR). Gene expression changes of androgen receptor were examined by using gel electrophoresis method. This study shows no significant difference of body weight and testis changes (P > 0.05) compared to negative control group. Gene expression changes are unsuccessful due to lack of RNA integrity and contamination of the DNA ladder. To improve the study, the samples need to be stored in correct temperature and optimize action of experimental procedure have to be done to reduce risk of contamination.