Phytochemical investigation of ethyl acetate extract from hedyotis diffusa / Anida Daud

Hedyotis diffusa is well known folk medicine in East Asia. There were not much studies have been carried out on this plant and very few articles were published on Hedyotis diffusa. Hedyotis diffusa was selected for this study due to its medicinal properties which in used by the locals in traditional medicine as a health tea. This study was done to investigate the phytochemical constituents present in the leaves of Hedyotis diffusa by extraction, fractionation and isolation of phytochemical constituents from the leaves of Hedyotis dif!usa leaves. Several steps were done in order to accomplish the objectives. Fractionation was done for crude ethyl acetate extract to separate compound(s) into different fractions. Then, isolation of compound(s) was done from the fractions using Preparative Thin Layer Chromatography (PTLC). In this study, cerric sulphate was used as spray reagent. Compound(s) of interest was then subjected to purification to obtain pure compound(s). The pure compound(s) undergoes several test methods to identify its structure by using spectroscopic technique, which was Nuclear Magnetic Resonance (NMR) through 1H and 13C-NMR analysis. Two (2) compounds were successfully isolated from this study but identification of the compounds had not yet completed.