Effect of various water on growth and secondary metabolites of 3PR3 and HAB10R12 fungi / Zalikha Hassan

The objective of this study was to determine the effect of minerals from different types of water and effect of different media on growth and production of secondary metabolites produced by two fungi strains; HAB10R12 and 3PR3. The study was performed by growing the pure culture of HAB10R12 and 3PR3 in both PDA and PDB by using four types of water; ultra purified water, Evian mineral water, TY Nynt mineral water and Sole mineral water for duration of three weeks and four weeks. Then, the myceliums of both strains were subjected to extraction process. The extracts obtained will be analyzed by using HPLC and the biological activity of the extracts will be determined by using disc diffusion method. From HPLC chromatogram analysis, both strains produced new secondary metabolites under the stress media. The extracts produce by both strains show negative antifungal activity against C.albican but show some positive antimicrobial activity against E.coli