Isolation of chemical constituents from methanolic extract of acanthus ebracteatus / Norsyazana Ab Hashim

Acanthus ebracteatus is traditionally used for the treatment of wide range of diseases. In Malaysia, the boiled seeds are commonly used as a cough remedy. The seed also used to treat boils. Acanthus ebracteatus was selected for this study due to its medicinal properties which have long been used as traditional folk medicine and not much study has been carried out on this plant. This thesis focused on investigation of chemical constituent from methanolic extract of Acanthus ebracteatus leaves. In order to accomplish the objective, several steps were done. First, an appropriate solvent system was selected and used for thin layer and column chromatographic techniques. TLC was performed on silica plate (alumina coated, silica gel 60 F254). Detection of compound using TLC was carried out at 240 nm and by eerie sulphate as spray reagent for UV inactive compound. For fractionation, Sephadex LH-20 column chromatography was used to separate compound into different fractions. Purity of compounds was checked on TLC with different percentage of solvent system using ethanol, ethyl acetate, and water. The pure compound(s) obtained undergoes several test methods to identify its structure by using spectroscopic technique which was Nuclear Magnetic Resonance (NMR) spectrometer through 1H NMR and 1H-NMR analysis was done. 13C-NMR analysis. Because of time limitation, only 1H-NMR spectrum was run on bruker spectrometers operated at 500 MHz. The structural elucidations were based on the analysis of physical and spectroscopic data. Two (2) compounds were successfully isolated from this study but identification of the compounds has not been yet completed and need further interpretation.