Developement of rapid PCR method for the detection of genetic polymorphism of CYP2C19 / Mohammad Izzuddin Halimi

Cytochrome P450 2C19 enzymes are predominantly found in the human liver, and have important functions in the metabolism of many different classes of commonly used drugs. Their genetic polymorphisms give rise to both important interethnic variability in metabolism and the risk of treatment failure or dose-dependent drug toxicity. The aim of this research is to develop a rapid PCR (polymerase chain reaction) based method to detect genetic polymorphism of CYP2C19 and to validate the PCR method that has been developed. The primers were designed according to the gene and followed by reconstitution of primer working stock. This research has a clinical significance as CYP2C19 polymorphism has been related to the usage of proton pump inhibitor drug that are usually used in the treatment of gastric or peptic ulcer patients. By getting the result from this research, we can know who is a poor metabolizer and who is rapid metabolizer and with this we can individualize each patient's own dosage regimen.