Development of a PCR based method for the detection of HLA-B*1502 (genotype marker for hypersensitivity of carbamazepine) / Puteri Huziana Hushairi

Polymerase Chain Reaction (PCR) is an amplification technique used to detect smaller quantities of DNA and beneficial in providing information for genotyping because they allow large numbers of sample to be processed in short time. HLA­ B*1502 has been associated with hypersensitivity reaction that occur in Asian patients treated with Carbamazepine. The aim of this research is to develop PCR based method in detection of HLA-B locus in patient with carbamazepine hypersensitivity and to validate the PCR method that has been developed. The primers were retrieved from journal and modified accordingly to gene sequence and were reconstituted. PCR and Gel electrophoresis to analyze PCR product has been done. Based on the result, detection of HLA-B*1502 can be achieved via PCR. Hence, detection of HLA-B*1502 using PCR is beneficial in early prevention of hypersensitivity reaction towards patients who receive carbamazepine treatment.