Synthesis of high molecular weight DNA ladder / Dayana Abdul Bari

Abdul Bari, Dayana (2009) Synthesis of high molecular weight DNA ladder / Dayana Abdul Bari. Degree thesis, Universiti Teknologi MARA (Kampus Puncak Alam).

Abstract

DNA ladder has been used as a tool in laboratories to determine the size of DNA fragment. The aim of this study is to synthesize high molecular weight DNA ladder and to optimize PCR reaction in order to obtain high yield and specific product. High molecular weight DNA ladder ranges from 0.5 - 10 kbp. DNA ladder is synthesized by using Polymerase Chain Reaction (PCR) method, where lambda phage DNA is used as a template for both PCR reaction and designing primer. The results showed that high molecular weight DNA ladder could be synthesized in laboratory setup. Thus, it could be used as a laboratory tool in the future and may reduce the cost incurred by the faculty in carrying out related studies.

Metadata

Item Type:	Thesis (Degree)
Creators:	Creators Email / ID Num. Abdul Bari, Dayana UNSPECIFIED
Contributors:	Contribution Name Email / ID Num. Thesis advisor Yusof, Rosmadi UNSPECIFIED
Subjects:	H Social Sciences > HD Industries. Land use. Labor > Special industries and trades > Pharmaceutical industry Q Science > QK Botany > Plant physiology > Botanical chemistry. Phytochemicals
Divisions:	Universiti Teknologi MARA, Selangor > Puncak Alam Campus > Faculty of Pharmacy
Programme:	Bachelor of Pharmacy
Keywords:	high molecular, DNA
Date:	2009
URI:	https://ir.uitm.edu.my/id/eprint/105530