Chromatographic study of vitex hexane extract / Noor Fadzila Badruddin

Traditional plants have been used in the medicinal practice since hundred years ago. Most of these plants were claimed to have great therapeutic activities against various diseases. This study is pertaining to the purification and identification of chemical constituents of Vitex trifolia (V trifolia, family: Verbenaceae). Many studies reported the potential biological activities of this plant. However, only few scientific evidences have proved all these claims and further investigation should be conducted to examine the chemical compositions of V trifolia. The literature search on the chemical constituents of Vitex species had concluded that flavonoid was the most common type of chemical constituent of Vitex species. The chromatographic study had been applied in order to purify and investigate the chemical constituents from the leaves of V trifolia hexane extract. Subsequently, spectroscopic evidences suggested that one of the non polar substances could be a labdane-type compound that can be used as a reference standard in quantitative measurements.