Chromatographic study of vitex methanol extract / Fia Iskandar

The methanolic extract of the leaves of Vitex trifolia was fractionated in order to obtain relatively polar compounds. Two compounds namely Compound A and Compound B were isolated and purified. They were subjected under 1H-NMR spectroscopy. The 1H-NMR spectrum of the compounds were analysed and compared with the findings from other journals. Compound A was suggested to have a methoxy, a phenol, a hydroxyl, a carboxylic, and an aromatic proton. Meanwhile, compound B may possess methoxy or carboxylic groups. Hydroxyl and carboxylic groups contributes to the polar characteristic of the compound. In addition, the literature review showed that the highly polar extracts of Vitex species have been found to be cytotoxic.