Collection, extraction, fractionation and isolation of phytochemical constituents from leaves of acanthus ebracteatus / Nurul Huda Mesbah

Nowadays, medicinal plants have gained the interest of many researchers from all over the world. In order to understand the plants and their beneficial constituents in details, many researcher from various disciplines now channeling their great concern to discover the benefits of these medicinal plants with an aid of modem medicinal equipments. However, in the case of the leaves of Acanthus ebractetus, the studies being made on it were very few and needed to be studied as wider as their benefits were traditionally approved can relief particularly inflamed joints effectively. By focusing on this research, the objective is to investigate the phytochemistry of Acanthus ebractetus. The leaves powder was soaked inn-hexane, dichloromethane, ethyl acetate and methanol at room temperature. The extracts were then being checked by using thin layer chromatography and the one containing interesting compound has been subjected to fractionation by using silica column chromatography. Fractions have been monitored through a thin layer chromatography (TLC) and the chemical composition of the crude extracts were analyzed and observed under UV light. The compound of interest has been subjected to NMR analysis for identification. There are 84 fractions that have been collected by fractionation using column chromatography and there are two compounds that have been identified. However, the structure of active ingredients in the leaves of Acanthus ebractetus was not elucidated.