NMR-based identification of carbamazepine metabolism through CYP2D6 enzyme / Norsuryahani Rahman

This project is aimed to identify metabolites of carbamazepine by action of CYP206 enzyme. Carbamazepine has numbers of side effects. It is believed that metabolites of carbamazepine are responsible for the occurrence of its side effects although the mechanism underlying the reactions is not known. Thus, this research project will investigate carbamazepine metabolism by using biological catalyst. Different concentrations of carbamazepine were used with different concentrations of CYP206 enzyme in two differents pH of media. After incubation, the reaction mixtures were extracted using chloroform. The residues were subjected to analysis using chromatographic and spectroscopic technique. The transformed metabolites of carbamazepine were purified through thin layer chromatography (TLC) and structure metabolites were elucidated through nuclear magnetic resonance (NMR) spectroscopy. As a result of carbamazepine metabolism by CYP206 enzyme, the metabolites being produced are 10, 11-dihydroxycarbamazepine and 10, 11-dihydroxy-10-hydroxycarbamazepine. Even though the compounds are not main metabolites in metabolism of carbamazepine as stated in various literatures, we managed to identify new potential compounds that may have possible contribution in various toxicity problem of carbamazepine.