Extraction and determination of the total phenolic content from different parts (leaves and stems) of spinacia oleracea by using water and ethanol / Noorafiza Fauzi

This study was carried out to determine the total phenolic content in spinach by using water and ethanol as the solvent for extraction. This study also compared the total phenolic content of the spinach leaves and stems. The total phenolic content was determined by using folin-ciocalteu reagent with the standard gallic acid measured at 725 nm using the Uv-Visible spectrometer. The total phenolic content of the spinach leaves in water extract was 0.8384 mg/g GAE while in ethanol extract was 0.7120 mg/g GAE. For the spinach stems, the total phenolic content in water extract was 0.4110 mg/g and 0.3848 mg/g in ethanol extract. From this finding, it shown that the phenolic content for both spinach leaves and stems in water extract is higher than ethanol extract. Besides that, it also shown that spinach leaves have higher total phenolic content than spinach stems.