Carbamazepine metabolism by CYP3A4: in vitro studies using NMR spectroscopy / Khalidah Maruan

The main objective of conducting this study is to identify the metabolites of carbamazepine metabolised by CYP3A4 using various concentrations of carbamazepine and CYP3A4 enzymes at different pH condition and investigate the metabolism through spectroscopic technique. A number of processes involved in order to achieve this objective. First process was enzymatic reaction of carbamazepine and followed by extraction and evaporation in order to isolate pure metabolites. The metabolites were further subjected to Thin Layer Chromatography technique for purification and structural characterization by 1H-NMR. Investigation through 1H-NMR identified the metabolic changes in carbamazepine nucleus and the resulting metabolites formed had been identified as 10, 11-dihydro- l 0-hydroxycarbamazepine and I 0, 11- dihydrocarbamazepine. Therefore, with the identification of metabolites formed, this study has accomplished its objective as stated above.