Preliminary study on phospholipids in marine fish species by using high-performance liquid chromatography / Nazathul Shima Azizi

The Burton et al. (1985) procedure was selected to identify and separate phospholipids in three marine fishes. The three marine fishes were herring, mackerel and sardine, which were selected among the most consumed fishes in Malaysia. Two different concentrations of SDS were used in the extraction system. The primary objectives were to separate and identify various phospholipids in the fishes as well as to compare the usage of two different SDS concentrations on the extraction system in term of total lipid yield. From this analysis, the most separated and identified phospholipids among the three fishes, were Phosphotidylethanolamine (PE) and Phosphotidycholine (PC). When extracting, it was found out that the lower the concentration of SDS used, it will give higher total lipids. In fish samples, the usage of different concentration in SDS solutions gave different detection of phospholipids. For 0.05M of SDS, PI, PE and PC can be identified in Herring, PE and PC in both Mackerel and Sardine. As comparison, 0.1M of SDS concentration will give the following results; only PE and PC can be detected in Herring, PE, whereas only PE and PC can be detected in both sample Mackerel and Sardine