Effect of supplementation of tocotrienols on sperm counts collected from male rat subjected to force swimming stress / Mohd Amizie Ibrahim

The study was conducted to determine the effect of tocotrienols to sperm counts in male rats which were subjected to force swimming stress. Twenty four adult male Sprague­ Dawley strain rats were randomly divided into four groups, each group consist of six rats. The control group (CG) which labeled as group A were fed normally and do not subjected to force swimming stress. Second group (group B) does not subject to force swimming stress but were received 0.2ml com oil orally. Third group (Group C) were subjected to force swimming stress two hours a day, for 30 days. Fourth group (group D) were subjected to force swimming stress and fed orally with 60mg/ml of tocotrienol. After 30 days of experiment the rats were sacrificed and the sperm samples were taken from epididymis cauda. Sperm sample were analyzed by using standard hemocytometer chamber. The result are the sperm count, percentages of dead sperm count and percentages of life sperm counts of male rats between the group B, group C and group D shows a significant different (p=0.00) with the group A. This experiment shows that intensive forced swimming stress can cause oxidative stress which may reduce the number and increase mortality rate of the sperm in male rats which can be protected with supplementation of the tocotrienol.