Effect of gynura procumbens aqueous extraction on interleukin-2 production in peripheral blood mononuclear cells / Nadirah Anis Che Abdullah

Immunomodulation is a process which can modify the immune system of an organism by intruding with its function. Medicinal herbs and their bioactive component had indicated to be a crucial source of immunomodulators. Research has proven that the herbal preparation from plant can be safer and effective rather than conventional medicines. Objective: In this study, we want to determine the effect of Gynura procumbens aqueous extraction on Interleukin-2 expression in Peripheral Blood Mononuclear Cells. Method: Three different doses (50, 200 and 800 μg/ml) of Gynura procumbens are cultured on PBMCs with or without inhibitors (CLI-095 and Polymyxin B sulfate) to determine the expression of the IL-2 using Luminex assay. Result: Result indicated Gynura procumbens aqueous extraction has immunomodulatory effect. Data was analyzed using one-way analysis of variance (ANOVA) to compare between control group and treated groups of different doses of Gynura procumbens were not significantly different (P >0.005), as well as co-treatment with inhibitors which analyzed using Independent sample t-test also shows not significantly different (P >0.005). Conclusion: Gynura procumbens aqueous extract has an immunomodulatory effect in PBMCs on expression of IL-2.