Abstract
Determination of weak D types may reduce the risk of unnecessary alloimmunization and also beneficial for proper utilization of Rh negative blood units. Present study aimed to determine the weak D type among non-Chinese blood donors from National Blood Centre (NBC) Kuala Lumpur by using Polymerase chain reaction with specific sequence primer (PCR-SSP). Out of 104 Rh negative blood donors, only one turn out to be positive for weak D, but undefined to be detected by selected kits. The phenotype for the India sample were dCce. Rh phenotyping of all sample consist of 96% dce, 2.9% dCce, and 0.9% dCcEe. In conclusion, due to time constrained for sample collection and the use of kits, the weak D type undetermined. Hence, it is recommended to prolong the duration of the samples collection and to use the different weak D variant kits or using real time PCR.
Metadata
Item Type: | Thesis (Degree) |
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Creators: | Creators Email / ID Num. Hilmi, Mohd Sodiq Rifaei UNSPECIFIED |
Contributors: | Contribution Name Email / ID Num. Advisor Kamarudin, Evana UNSPECIFIED |
Divisions: | Universiti Teknologi MARA, Shah Alam > Faculty of Health Sciences |
Programme: | Bachelor of Medical Laboratory Technology (Hons) |
Date: | 2016 |
URI: | https://ir.uitm.edu.my/id/eprint/101207 |
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