Determination of tumor necrosis factor - α level in peripheral blood mononuclear cells treated with gynura procumbens / Mohammad Syazwan Kamaludin

Gynura procumbens is plant come from family of Asteraceae and is an annual evergreen shrub that grows plenteous in Southest Asia area such as Malaysia, Thailand and Indonesia. G. procumbens is traditionally used for treatment of kidney disease, cancer, rash, constipation, migrains, hypertension, eruptive fevers and diabetes mellitus. The following project was conducted to study the determination of Tumor Necrosis Factor – α (TNF – α) level in Peripheral Blood Mononuclear Cells (PBMC) when treated by using G.procumbens. G.procumbens leaf was extracted by aqueous and ethanolic method with different concentration (50 μg/mL, 200 μg/mL and 800 μg/mL). Fresh human blood then layered with Ficoll using density gradient separation segregation method to separate the red blood cells and white blood cells. Then at cell culture method, G.procumbens ethanolic and aqueous extraction and PBMC will be added to different comparing group. RPMI used as a media, Polymyxin B as a LPS inhibitor and CLI 0.95 acts as TLR4 inhibitor. The level detection of TNF – α was determined by using Luminex® Magnetic Assay. Even though, comparing with aqueous and ethanolic extraction with different concentration (50 μg/mL, 200 μg/mL and 800 μg/mL), there was no exact value in the expression of TNF – α when detection by using Luminex® Magnetic Assay. For that reason, statistical analysis by using SPSS method was unnecessary to be calculated. The result proved that G.procumbens is safe to be consumed by humans because G.procumbens does not show effect on the production of TNF – α because it is the one of the pro-inflammatory cytokines which is important in signaling and promote systemic inflammation. ). In contrast, G.procumbens exhibit in function as TNF – α inhibitor while TNF – α is vice versa.