The effects of sub-acute exposure of HAB10R12 ethanolic extract on the expression of N-methyl-D-aspartate receptor-I subunit gene in mice brain / Norhidayah Kasiman @ Kamisan

NMDA-Rl subunit is important in synaptic plasticity and learning process. The aim of the present study was to determine the effect of HAB10R12 extract on the expression of N-Methyl-D-Aspartate Receptor-I subunit gene in mice brain after sub-acute exposure of HAB10R12. Thirty mice were randomly divided into five groups which consist of normal saline, memantine, 5 mg/ml/kg, l O mg/ml/kg and 20 mg/ml/kg of HAB10R12 treatments. All the groups of mice were treated by intraperitoneal (IP) administration for 14 days. The mice were sacrificed at day I 5 and the brains were removed. RNA was extracted from the brains by using SY Total RNA Isolation System (Promega). QuantiFast SYBR Green RT-PCR Kit (Qiagen) was used to quantify the expression of NMDA-R1 subunit gene by using comparative threshold method that was recorded by Rotor-Gene 600 Series Software I. 7. The result showed that administration of 5mg/ml/kg HAB10R12 dose caused up-regulation of NMDA-R1 subunit gene in mice. In contrast, administration of 10mg/ml/kg HAB10R12 dose to mice cause down­ regulation of NMDA-R1 subunit gene. In conclusion, HAB10R12 might affect the expression ofNMDA-R1 subunit gene in mice brain after sub-acute exposure.