Abstract
Research work was aimed at improving vaginal smear technique for visualizing and recognizing cells types present during estrous cycle phase of female Wistar Kyoto rat (WKY) and to confirm that the reproductive hormones in those rats are influenced by phases of estrous cycle. Results showed staining with eosin and hematoxylin improved visual readability of cells compared to unstained smears. The length of estrous cycle in normal WKY rats was determined to be 5.02 ± 0.17 days with duration for proestrus (1.08 ± 0.04 days), estrus (1.27 ± 0.08 days), metestrus (1.53 ± 0.06 days), and diestrus (1.12 ± 0.06 days). The proestrus/estrus phases significantly increased estradiol levels (326.78 ± 100.00 pg/ml) compared to metestrus/diestrus (137.22 ± 38.42 pg/ml). There were no differences in luteinizing hormone (LH) and progesterone levels between the proestrus/estrus and metestrus/diestrus stages.
Metadata
Item Type: | Thesis (Degree) |
---|---|
Creators: | Creators Email / ID Num. Md Ali, Masliza UNSPECIFIED |
Contributors: | Contribution Name Email / ID Num. Thesis advisor lguegbe, Aloysius Umelo UNSPECIFIED |
Subjects: | Q Science > QH Natural history - Biology > Reproduction R Medicine > RM Therapeutics. Pharmacology R Medicine > RS Pharmacy and materia medica |
Divisions: | Universiti Teknologi MARA, Selangor > Puncak Alam Campus > Faculty of Pharmacy |
Programme: | Bachelor of Pharmacy |
Keywords: | estrous cycle, reproductive hormone |
Date: | 2008 |
URI: | https://ir.uitm.edu.my/id/eprint/100886 |
Download
100886.PDF
Download (1MB)