Enhancement of cytotoxic metabolite production from HAB10R12 an endophyte / Nor Ashiqin Aiman Roslan

Natural products have played an important role in treating cancer by serving as compounds in their natural form or as templates for synthetic compounds. In the recent years, endophytes hold a new source of novel cytotoxic drug discovery. The endophyte HAB10R12 used throughout this study was isolated from a local medicinal plant. Preliminary screening found this endophyte to possess cytotoxic activity against P388, a murine leukaemic cell line. The aim of this study was to determine the effect of different culture conditions on the cytotoxic metabolite production of HAB I OR 12 against human colon (HCTl 16) and breast (MCF7) cancer cell lines. The various fermentation parameters include type of media (agar vs. broth), water source (tap water vs. deionized water), incubation time ( 1, 2 and 4 weeks) as well as strength of media ( full media strength vs. one fifth media strength). The sensitivity of HAB10R12 extracts encoded PDAl, PDA2, PDA4, PDB2(B), PDB2(M), TPDA, TPDB(B), TPDB(M), l/5PDA, l/5PDB(B) and l/5PDB(M) were determined against HCTI 16 and MCF7 cell lines. All the extracts were evaluated for in vitro cytotoxic activity against HCTI 16 and MCF7 cell lines using the MTT assay. The sensitivity of each cell line was characterized by the IC50 value. The result of this study indicated that endophytic fungus HAB10R12 incubated for 4 weeks and that prepared by using full strength medium with deionized water to possess stronger cytotoxic activity when compared to other fermentation parameters. The endophytic fungus HAB10R12 with its promising cytotoxic activity has the potential to be developed as cytotoxic drug.