Total phenolic content and radical scavenging activity from the leaves of pogostemon cablin / Siti Munira Yahaya

Pogostemon cablin belongs to Lamiaceae family. It is widely distributed in Malaysia, China, India and Brazil. Pogostemon cablin is also known as patchouli. The main objectives of this research were to screen the presence Alkaloids, Flavanoids, Saponins and Terpenes and also to determine Total Phenolic Content (TPC) and radical scavenging activity from the leaves of Pogostemon cablin extract. The solvent used for the extraction were methanol, chloroform and hexane. From this study it was found that the leaves of Pogostemon cablin contain alkaloids, flavanoids and saponins and the major compound presence was alkaloids. Standard calibration curve of gallic acid was used in order to determine the total phenolic content. There is a linear correlation between concentration and absorbance of gallic acid. As the concentration of the gallic acid increases, the absorbance also increases. In TPC method, result shows that hexane extracts have the highest amount of phenolic content followed by methanol extract. While, chloroform extracts have the least amount of phenolic content. Antioxidant activity of the extract was determined by using DPPH radical scavenging method. Methanol extract shows the highest % scavenging activity while chloroform extract was the least scavenging activity. As the conclusion, hexane and methanol have the highest phenolic content and scavenging activity respectively.