Phytochemical screening, antioxidant and antibacterial activities of citrus hystrix leaves / Syafiqah Alya Samlin

Samlin, Syafiqah Alya (2017) Phytochemical screening, antioxidant and antibacterial activities of citrus hystrix leaves / Syafiqah Alya Samlin. [Student Project] (Unpublished)

Abstract

Phytochemical screenmg, antioxidant and antibacterial activities, Citrus hystrix leaves have been studied. Different polarities of extraction solvents are used which are hexane, chloroform and ethanol. Ethanol crude gave the highest percentage yield (3.14%) followed by chloroform and hexane. Secondary metabolites which are flavanoid, alkaloid, sterol, steroid, tannin, phenol, terpenoid and glycoside are presence in ethanol crude extract except saponin. Antibacterial activities against Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Salmonella typhi were conducted. Disc diffusion method gave a result of highest inhibition zone by hexane crude extract against S. aureus ( 17 mm) while E.coli has no inhibition zone against chloroform and ethanol. TLC analysis conducted by using hexane and chloroform as the combination of solvent system with ( 1 :9) ratio gave the best separation of
compound under long (365 run) and short (254 run) wavelength of UV lamp. The most antioxidant compounds were traced in ethanol crude extract by using thin layer
chromatography (TLC) bioautography.

Metadata

Item Type:	Student Project
Creators:	Creators Email / ID Num. Samlin, Syafiqah Alya UNSPECIFIED
Subjects:	Q Science > QD Chemistry Q Science > QD Chemistry > Organic chemistry > Oxidation Q Science > QD Chemistry > Extraction (Chemistry)
Divisions:	Universiti Teknologi MARA, Negeri Sembilan > Kuala Pilah Campus > Faculty of Applied Sciences
Keywords:	Phytochemical screening, antioxidant, and antibacterial activities of citrus hystrix leaves, UiTM Negeri Sembilan
Date:	2017
URI:	https://ir.uitm.edu.my/id/eprint/26076