Differential pulse anodic stripping voltammetric (dpasv) technique for reactive black 5 analysis at glassy carbon electrode / Nur Aqilah Abdul Rahman

Dyes are generally defined as an aromatic organic compound which shows an
affinity towards the substrate to which they are being applied. Dyes are used to give
colour to fabrics. Hence, the amounts and contents of dyes that used in fabrics
industry must be analyzed. The presence of dyes in wastewater samples at any level
is not safe for human. Therefore, dyes analysis needs a sensitive, precise, rapid,
accurate, simple and low cost analytical method for dyes determination. The
differential pulse anodic stripping voltammetry (DPASV) technique using glassy
carbon electrode (GeE) as a working electrode and phosphate buffer at pH 4.2 as a
supporting electrolyte has been proposed to be developed. The experimental
voltammetric parameters were optimized in order to obtain a maximum response
with analytical validation of the technique. The optimum parameters were initial
potential <Ei) = +0.3 V, end potential (Ef) = +1.0 V, scan rate (v) = 0.04 V/s,
accumulation time (tacc) = 50 s, accumulation potential (Eacc) = 0.4 V and pulse
amplitude = 0.075 V. The anodic peak was appeared at 0.77972 V. The curve was
linear from 0.5 to 1.25 mg/L (R2=0.9986) with detection limit of 0.025 mg/L. The
precisions in terms of relative standard deviation (RSD) were 0.08%, 0.62% and
0.50% for consecutive three days. The range recovery achieved for 0.5, 0.7 and 1.0
mg/L of RB5 standard solution in simulation dye sample was 104.40%, 89.71%, and
111.15% respectively. It can be concluded that the developed technique is precise,
accurate, rugged, low cost, fast and has potential to be an alternative method for
routine analysis of RB5 in stimulation dye sample.