LC profiling and determination of stevioside and rebaudioside a in ten stevia rebaudiana bertoni accessions / Amanina Alwani Arshad

Stevia rebaudiana Bertoni leaves contain non-caloric natural sweet compounds which are steviol glycosides. Stevioside and rebaudioside A are types of steviol glycosides that are the most abundant in the leaves. The aims of this study are (1) to characterize and evaluate morphological characteristics of ten stevia accessions. (2) to obtain an LC-MS profiling of stevia leaves extract and quantify major constituents of steviol glycosides and identify other major compounds that presented in the stevia leaves extract (3) to determine the effect of different harvesting stages towards stevioside and rebaudioside A.