Determination of caffeine, chlorogenic acid and nicotinic acid in coffee beans by using HPLC / Nor Hanisah Mat Yassin

Mat Yassin, Nor Hanisah (2008) Determination of caffeine, chlorogenic acid and nicotinic acid in coffee beans by using HPLC / Nor Hanisah Mat Yassin. [Student Project] (Unpublished)


Coffee usually comes in two types of coffee beans. They are coffee arabica and
coffee robusta. These coffees contain many chemicals that are affected during the
roasting process. The coffee contains caffeine, chlorogenic acid and nicotinic acid
which give a variety of the effect that is both beneficial and harmful to human.
This research will determine the amount of compound such as caffeine,
chlorogenic acid and nicotinic acid present in coffee after roasting. The coffee
beans were roasted in an oven and grounded coffee was brewed in freezer. The
sample preparation was quite simple involving only heating water extraction and
filtration. Analytical HPLC unit consists of Water 717 plus Autosampler and
Water 600 Controller, Water 2487 Dual λ Absorbance detector, a 150 x 4.6 mm
i.d. Merck Superspher 100 Reversed Phase (RP), C-18 column with 5 μm particle
sizes. The analysis is uses reversed phase (RP) column with gradient elution of 10
mM citric acid (A) and methanol (B). Robusta coffee beans contained the highest
amount of caffeine compared with arabica were, 36.0290 mg/ml and 35.2950
mg/ml respectively. The amount nicotinic acid in roasted coffee arabica for five
minute was 0.5859 mg/ml and roasted coffee robusta was 0.7000 mg/ml. The
amount of chlorogenic acid in brewed coffee samples is smaller than roasted
coffee sample. In roasted coffee sample, the amount of CGA in arabica and
robusta were 10.8936 mg/ml and 15.1398 mg/ml respectively.


Item Type: Student Project
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Mat Yassin, Nor Hanisah
Divisions: Universiti Teknologi MARA, Shah Alam > Faculty of Applied Sciences
Keywords: Caffeine, chlorogenic acid, nicotinic acid, coffee beans, HPLC, chemical
Date: 2008
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