Extraction and comparison of the total phenolic content from Malaysian grown kalanchoe pinnata (leaves and petioles) by using two different solvents ; Water and Ethanol / Fatimatuzzahraa Mohd Fadzel

Nowadays, even if technology has improved, humans still need the natural
resources such as herbs which serve as a traditional remedies since so many
years ago. There are many natural resources with high total phenolic content
which are antioxidants constituents. From the previous research, antioxidants
were found in tea leaves , garlic, broccoli and mangosteen However, there are
no research of total phenolic content or antioxidant on a Malaysian-grown
kalanchoe pinnata. The objective of this study is to extract and determine the
total phenolic content in two parts of kalanchoe pinnata; leaves and petioles in
two different solvents ; water and ethanol. The sample were extracted and
Total phenolic content was determined by using Folin-Ciocalteu assay based
on the Gallic acid standard calibration curve by using UV-VIS Spectrometer
at 765nm. The Total phenolic content value was shown to be higher in leaf
that were extracted by using ethanol with the value of 0.8816 mg/g GAE . As
a conclusion, the content of total phenolic content in kalanchoe pinnata leaf
are more effectively extracted by using ethanol than compared to water.
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