Synthesis, characterization, toxicity of silver nanoparticles reduced by ethanol extract of Beijing grass / Syazwani Abd Rahman and Dr. Ainorkhilah Mahmood

Recently, with arising concern in regards with some drawbacks from chemical and physical methods to synthesize silver nanoparticles (AgNPs), plant mediated silver nanoparticles (AgNPs) have garnered such a considerable attention from many researchers due to its beneficial approach that are environmental in nature as well as the attractiveness of its cost-cutting approach in which the established method from chemical and physical are pale in comparison to biological approach. In this present study, the ethanol extract of Beijing Grass will acts as bio-reducing agent for the synthesis of AgNPs where the setting parameter such as concentration and volume ratio in this research to find the optimized parameter for BGE-AgNPs. The optimized parameter are set up from 0.5 mM to 5.0 mM with volume ratio AgNO3 from 9:1 and 5:5. The assynthesized AgNPs are then characterized using UV-Vis Spectroscopy in the range of 400 nm - 800 nm. The results showed a successful synthesized via biological approach utilizing the parameter mentioned, where basing on the UV-Vis absorption spectra, a distinguishable peak at 469 nm for optimized concentration of 2 mM silver ion (Ag+) and at volume ratio 9:1 are observed. Next, a schematic review highlighting on in-vitro cytotoxicity effect of aquatic species are conducted as the concern of rapid proliferation of AgNPs aligned to exponential growth of increasing demand in many industries could potentially be a risk if release to environment.