The effects of bevacizumab on human Tenon’s fibroblasts: an in vitro study / Nurul Nabila Baharudin

Purpose: To understand the effect and mechanism of bevacizumab in inbiting wound healing on primary human Tenon's capsule fibroblasts (HTF) in vitro. Methods: HTF was cultured in RPMI media and bevacizumab was added to study the effect of bevacizumab on HTF in vitro at a concentration of 0.25,1.25,6.25 and 12.5 mg/mL. The HTF morphology was identified using vimentin antibody and DAPI staining under immunofluorescent images. MTT assay was done to assess fibroblast cells viability and cell death. The results were compared with mitomycin C.